Cox regression models were fitted to determine the associated factors on survival. Human BC cells (SKBR-3) could be differentiated from normal blood based on the multiple light scatter and cell surface Ceftiofur
marker expression by multiparameter flow cytometry. The method was found to have a sensitivity limit of 10?5 and was effective for detecting human BC cells in vivo. It also found that this method had a higher specificity compared with RT-PCR. For the retrospective study, the median OS was 95 weeks and 65.5 weeks (P < 0.05, 2-tailed) for patients with CTCs <5 and CTCs ��5, respectively. Kaplan-Meier was used to analyze the patients' survival with Log Rank P = 0.004 and Breslow P = 0.003, which showed that these two groups had statistically significant difference. Cox regression analysis was performed, and we found CTCslevels, metastasis <a href="http://www.selleckchem.com/products/3-methyladenine.html
">www.selleckchem.com/products/3-methyladenine.html and age (P < 0.05) were three relative factors for patients' survival. Multiparameter flow cytometry can detect CTCs effectively and has the potential to be a valuable tool for prognosis assessment among BC patients in clinical situations in China. ? 2010 International Society for Advancement of Cytometry Breast Cancer (BC) is the most common cancer in women in developed countries. In developing countries, such as China, the incidence of BC is currently increasing, particularly in the larger cities (1). In many patients with solid tumors of epithelial origin, circulating cells with the characteristics of tumor cells can be identified in the peripheral blood that is known as circulating tumor cells (CTCs). These cells are present not only in patients with metastatic disease but also in those whose tumors are apparently localized (2). There may be intermittent shedding of tumor cells into the circulation corresponding with micro-invasive events within the tumor. The first phase of the metastatic cascade consists of loss of tumor cell adhesion, induction of cell motility, and local tumor cell invasion (3). These steps are followed by either dissemination to regional lymph nodes or circulation through the blood, and homing to secondary organs, where the tumor cells may reside as viable cells in a ��dormant�� state (4). Some of these cells eventually become precursors of metastases Selleckchem Sunitinib
that can arise many years after curative resection of the primary tumor (5). Although apoptosis contributed to a high rate of circulating cells, only a small part of the cells can adhere in organs through blood vessels that were named as circulating tumor stem cells (CTSCs) (6). CTCs cells can be selected with the fluorescent antibody that linked to a monoclonal antibody directed against CD45 for negative selection of leukocytes (7�C9). From these cluster cells, Ep-CAM (epithelial-cell adhesion molecule) and Cytokeratin 8,18,19 (Cytokeratin 8,18,19-phycoerythrin staining) positive cells are the target cells as these two monoclonal antibodies can be adhered in epithelial cells.