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15 BGB324 Myths Uncovered

05) higher than were those observed in extended semen or frozen-thawed spermatozoa supplemented with GSH. However, at that time, frozen-thawed semen supplemented with 2?mm GSH presented significantly higher (p?<?0.05) levels of free cysteine radicals than did extended semen (FT C: 8.5?��?0.6 vs. FT GSH: 4.4?��?0.3). DNA fragmentation levels were low in all treatments after 30 and 240?min of incubation at 37?��C. However, whereas extended semen and frozen-thawed spermatozoa supplemented with 2?mm GSH did not differ after incubation at 37?��C for either 30 or 240?min, frozen-thawed control semen presented significantly (p?<?0.05) higher percentages of spermatozoa with fragmented DNA at 240?min post-thawing when compared with extended and frozen-thawed semen supplemented with 2?mm GSH (Fig.?2). Freeze-thawing <a href="http://www.selleck.cn/products/BIBF1120.html">Nintedanib of boar spermatozoa significantly (p?<?0.05) reduced the percentages of viable spermatozoa both after 30 and 240?min post-thawing (Fig.?3). Nevertheless, the extent of this damage differed between frozen-thawed control and frozen-thawed semen supplemented with 2?mm GSH, as the former presented significantly lower percentages of viable spermatozoa than the latter, both after 30 and 240?min post-thawing (FT C: 33.5?��?1.4 vs. FT GSH: 46.9?��?1.9). Table?1 shows (as mean?��?SEM) the percentages of acrosome-intact spermatozoa in extended semen, frozen-thawed control and frozen-thawed supplemented <a href="http://www.selleckchem.com/products/dinaciclib-sch727965.html">SCH727965 in vitro with GSH after 30 and 240?min of incubation at 37?��C. Again, freeze-thawing of boar spermatozoa was seen to significantly (p?<?0.05) decrease the percentage of acrosome-intact spermatozoa. <a href="http://www.selleckchem.com/products/r428.html">BGB324 datasheet Notwithstanding, this reduction was significantly higher (p?<?0.05) in frozen-thawed control than in frozen-thawed semen supplemented with 2?mm GSH (e.g. at 240?min post-thawing, FT C: 20.9?��?1.0 vs. FT GSH 38.8?��?1.6). After 30?min of incubation at 37?��C, the percentage of viable spermatozoa with high peroxide levels (DCF+/PI?) was found to be slightly but significantly higher (p?<?0.05) in frozen-thawed control than in extended and frozen-thawed semen supplemented with 2?mm GSH (Table?1). In contrast, the percentage of spermatozoa with high intracellular superoxide levels (E+/YO-PRO-1?) was not found to be affected by sperm cryopreservation or GSH supplementation. Table?1 also shows data on total and PMOT in extended semen, frozen-thawed control and frozen-thawed spermatozoa supplemented with 2?mm GSH. As expected, boar sperm cryopreservation significantly decreased total (TMOT) and PMOT. However, in both parameters, the reduction observed in frozen-thawed control was significantly (p?<?0.05) higher than that observed in frozen-thawed spermatozoa supplemented with 2?mm GSH, both at 30 and 240?min post-thawing (e.g. PMOT at 240?min post-thawing, FT C: 18.0?��?1.1 vs. FT GSH 37.2?��?2.0). Table?2a shows the correlation coefficients among all the eight sperm parameters evaluated at 30?min.</div>
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