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Disguised Solutions To AZD2281

2011). The data processing Wortmannin purchase results were summarized in Table?2. Clustering of the HQ tags generated a reference data set composed of 64?006 tags, of which the 13?953 that were consistently expressed across all 18 samples were used for differential expression analysis and gene network construction. Of the 13?953 unitags, 7089 could be mapped to the isotigs obtained by assembling 454 transcriptome data (Wang et?al. 2013). Among the unitags mapped to isotigs, 6937 (97.86%) were unambiguously mapped to single isotigs, suggesting that these unitags can represent the matched isotigs. The remaining 152 (2.14%) were mapped to multiple isotigs, which could be because of the existence of conserved domains shared by members of multigene families. The relatively low mapping rate (~50%) could be because of the incompleteness of the transcriptome assembly. Among the 6937 uniquely mapped unitags, 3869 could be annotated, including 3394 obtained by BlastX against the Swiss-Prot database and 475 by tBlastX against the Nt database, resulting in a total annotation rate of 56%. Differential expression AZD2281 chemical structure analysis showed that a large number of unitags were differentially expressed after acute heat stress. Specifically, 857, 895 and 1038 DETs were found at 3-h, 6-h and 1-day post-heat exposure, respectively (Fig.?1a). For chronic heat stress (3, 7 and 14?days), the number of DETs maintained within the range of 320�C350 (Fig.?1a) Hesperadin across all time points, suggesting that chronic heat stress induced a smaller number of DETs than acute heat stress. A Venn diagram revealed that a total of 2137 unitags showed differential expression after acute heat stress, accounting for 15.32% of the unitags detected, whereas only 767 (5.50%) were differentially expressed after chronic heat stress (Fig.?1b). Altogether, 2521 unitags showed differential expression after heat stress, of which only 21 were differentially expressed in all 6 time points of heat stress (Table S1, Supporting information). As shown in Fig.?1c, in comparison with the background, four GO terms were significantly enriched (EASE <0.05) in acute heat stress DETs, including biosynthetic process (GO: 0009058), response to stress (GO: 0006950), macromolecule metabolic process (GO: 0043170) and primary metabolic process (GO: 0044238), whereas only biosynthetic process (GO: 0009058) was significantly highly represented (EASE = 2.65E-2) in chronic heat stress DETs. No GO terms were found to be significantly differentially distributed between acute and chronic heat stress. We tested the expression of seven DETs at different time points using qRT�CPCR.</div>
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