37,38 The CD4+ cell count was determined by flow cytometry, and plasma HIV-1 RNA load was measured by a quantitative reverse transcriptase polymerase chain reaction, which has a lower limit of detection of 50?copies/mL. Statistical analysis was performed using SPSS version 17.0 software (SPSS Inc, Chicago, IL). All probabilities Gamma-secretase inhibitor
were two tailed and P values <.05 were regarded as significant. Data were described as mean and standard deviation (SD) for quantitative variables and compared using the Student t test or the Mann-Whitney test as appropriate. Kruskal-Wallis test was used for the comparison between the 4 groups of fat distribution. Categoric variables were described as counts and proportions and compared using the chi-square <a href="http://www.selleckchem.com/products/Adriamycin.html
">selleckchem or Fisher exact test. Odds ratios (ORs) and respective 95% confidence intervals (95% CIs) were computed using unconditional logistic regression for estimating the association between hypertension and anthropometric measures and insulin resistance. In this sample of 364 HIV-1�Cinfected patients (250 men and 114 women) taking antiretroviral therapy (cART), 59.1% patients presented with CL. Table?I shows the characteristics of the study sample according to the presence of CL. Patients with CL were more frequently male, older, had longer HIV infection duration and length of cART, and presented a lower mean of all the anthropometric measures, with the exception of height and neck circumference. In addition, patients with CL had higher FMRs. Patients with CL had a significantly higher mean CD4+ cell count and viral suppression rate. No differences were observed between patients with and without CL regarding the cART regimens. BML-190
Also, no differences between patients with or without CL were observed regarding the frequency of antihypertensive medication use. Of the 364 patients, 34.3% were hypertensive. No significant differences were observed regarding the prevalence of hypertension in patients with and without lipodystrophy, regardless of sex or the definition that was used (Table?II). In addition, no differences in mean systolic BP (SBP) or diastolic BP (DBP) between patients with or without lipodystrophy were found (Table?I), defined either by clinical criteria or by FMR (SBP: with lipodystrophy 123.7?mm?Hg [16.8] vs without 119.4?mm?Hg [18.3], P=.068; DBP: with lipodystrophy 76.8?mm?Hg [9.9] vs without 76.0?mm?Hg [12.5]. P=.411). When patients were classified into 4 categories of fat distribution as previously described, the presence of hypertension was more prevalent in patients with isolated central fat accumulation and mixed forms of lipodystrophy (patients with abdominal prominence regardless of the presence or absence of lipoatrophy) both in men and in the total sample (Table?III). Patients with hypertension were older; had higher mean weight, body mass index (BMI), waist, arm, and neck circumferences; and higher waist/hip circumference ratio.