The chi-square test with Yates�� correction or Fisher��s exact test was used for comparisons between groups, as appropriate. Differences were taken as statistically significant if the P-value was <0��05. Escherichia coli strains were isolated from faecal specimens obtained from 240 of 250 (96��0%) diarrhoea children and from all 150 control subjects. The number of E.?coli colonies per patient varied from one to eight (mean?=?3��7). All isolated E.?coli colonies were tested for the presence of elt and est. elt and/or est were found in 60 E.?coli strains that <a href="http://www.selleckchem.com/JAK.html
">selleck chemicals llc were obtained from stools of 28 (7��2%) children, 22 (9��2%) with and six (4��0%) without diarrhoea. No statistical association between ETEC and diarrhoea was observed. The mean number of positive ETEC colonies per patient was 2��1. Mixed infection by elt-positive and elt-positive/est-positive Tenofovir
strains was identified in only one (4��5%) 10-month-old child with diarrhoea (Table?1). This patient was excluded from further analysis. The proportion of diarrhoea episodes associated with the excretion of ETEC showed seasonal variations, with the highest levels of organism isolation occurring in the rainier months of the year (P?<?0��00003, OR?=?13��4, 95% CI from 3��3 to 118��1). In fact, ETEC was isolated in the dry season only in 2004. Although elt-positive strains were more commonly detected in the rainy season, no statistical difference was observed. ETEC infection was scanty in children aged more than 24?months and was not associated with gender. elt-positive strains are statistically more common than the other ETEC genotypes in children aged from 2 to 3?years when compared with the other age ranges (P?=?0��0036, OR?=?30��0, 95% CI from 1��93 to 1493��2). Overall, CF genes were identified in 53��3% (32/60) of the ETEC strains isolated from 19 (67��6%) children, 15 (78��9%) with diarrhoea and four (21��1%) controls. All tested CF were detected. The most common CF type was CFA/IV, identified in 26 strains isolated from 14 (73��7%) children (Table?2) followed by CS14, detected in 14 strains obtained from <a href="http://www.selleckchem.com/products/napabucasin.html
">www.selleckchem.com 10 (52��6%) subjects. In six children with non-mixed infection, ETEC isolates expressing different CF simultaneously were identified, five (83��3%) and one (16��7%) of them harbouring est or elt, respectively (Table?2). CF detected in ETEC isolates presenting an identifiable CF were almost equally associated with elt-positive (8/44��4%) and est-positive (7/38��9%) strains (Table?2). The proportion of CFA/II-positive ETEC isolates harbouring CS1, CS2 and CS3 genes were similar. On the other hand, CS4?+ CS6 combination was the most common (7/53��8%) in CFA/IV-positive ETEC strains (Table?2). Year-to-year variations in the recovery of ETEC isolates harbouring CF genes were noted but no statistical difference was observed.