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Detailed Comments To Pembrolizumab In Note By Note Order

Incubation of mTCP3 seedlings in the presence of NAA did not result in increased activity of the pDR5::GUS reporter (Figure?3d,g,h), in contrast with the significantly elevated expression of this reporter in wild-type (Figure?3d,e,f) and TCP3SRDX plants (Figure?3d,i,j). These results suggest that expression of mTCP3 reduces auxin transport capacity and/or sensitivity. Membrane-localized PIN proteins are known to mediate polar auxin transport, and visualization of these proteins Crizotinib fused to GFP allows detailed characterization of their distribution and abundance (Teale et?al., 2006). To further define the effect of expressing mTCP3 on auxin transport, functional pPIN1::PIN1-GFP and pPIN2::PIN2-GFP constructs were introduced into mTCP3 plants. In comparison to wild-type roots (Figure S4a), PIN1 showed an overall decreased abundance but an unaltered localization pattern in mTCP3 roots (Figure S4b). By contrast, the PIN2 localization and abundance in mTCP3 roots (Figure S4d) was similar to that observed in wild-type roots (Figure S4c). These observations are consistent with a previous report showing that PIN1 localization selleck products and abundance were flavonol-sensitive but PIN2 localization and abundance were not (Peer et?al., 2004). The reduced abundance of PIN1 therefore probably accounts for reduced auxin transport capacity observed in mTCP3 plants. Microarray experiments were performed using ATH1 GeneChips, and transcriptome comparison between wild-type, mTCP3 and TCP3SRDX seedlings revealed a large number of de-regulated genes that were grouped into 11 functional categories (308 and selleckchem 75 genes for mTCP3 plants and 126 and 208 genes for TCP3SRDX plants were up- and down-regulated, respectively; Figure S5a�Cd). Expression of mTCP3 affected transcription of genes in the categories ��flavonoid biosynthesis�� (3% up-regulated; Figure S5a) and ��auxin signaling�� (9% down-regulated; Figure S5b). In contrast, among the up-regulated genes in TCP3SRDX plants, no flavonoid biosynthesis-related genes were identified, but 2% belong to the category ��auxin signaling�� (Figure S5c). Among the down-regulated genes in TCP3SRDX plants, no auxin signaling-related genes were detected, but 2% of the genes fall into the category ��flavonoid biosynthesis�� (Figure S5d). To explore potential TCP3 target genes that contribute to observed alterations of flavonoid accumulation and auxin response, further analysis focused on candidate genes that were associated with flavonoid metabolism and auxin signaling. Nineteen genes showing a more than twofold expression change (P?<?0.05) between wild-type and mTCP3 seedlings were identified (Table?1). Ten genes involved in the flavonoid biosynthetic pathway were up-regulated 3.27- to 24.35-fold in mTCP3 plants (Table?1).</div>
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